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Cell Biolabs彗星实验试剂盒

由于环境因素和细胞内的正常代谢过程造成的DNA损伤,每个细胞每天都会发生1,000到1,000,000个。虽然这些只占人类基因组约60亿个碱基中的一小部分,但如果关键基因损伤未及时修复可能会阻碍细胞的正常生理功能,进而增加癌变可能。彗星实验,或称单细胞凝胶电泳(Single cell gel electrophoresis,SCGE是一种测量单个细胞DNA损伤的常用技术。其原理简单,即在电泳场,将受损细胞DNA(包含片段和链断裂)与完整的DNA分离通过显微镜观察到损伤细胞呈现出典型的彗星状尾巴,然后通过测量计算彗尾大小对比出细胞DNA损伤的程度因为彗星实验特点,该方法几乎被用来评估任何类型的真核细胞的 DNA 修复能力,包括双、单链断裂的不同的 DNA 损伤情况。是一种能快速、大通量检测真核细胞DNA损伤进而判别遗传毒性的技术。

彗星实验结果图

彗星实验原理简单,但操作繁琐,需要丰富的实验经验和技巧,尤其常常出现的“脱胶”问题,困扰了科研人员除此之外有时为了跑出完美的“彗星”图案放在paper里,还需要重复做许多次实验,费时费力。为了解决上述问题,我们推荐CellBiolabsOxiSelectTMComet Assay Kit即彗星实验试剂盒来检测细胞的DNA损伤。该试剂盒不仅能让彗星实验化繁为简,还两种不同规格(3孔和96孔)的细胞电泳凝胶板供选择让少量样本和大量样本的DNA损伤检测通通轻松hold住。用该试剂做彗星实验流程如下图。

除了操作简便,OxiSelectTMComet Assay Kit还有以下优点:

1)      适用于各种DNA损伤检测,是一款非常好用的DNA损伤检测筛选工具;

2)      试剂盒中的载玻片经过特殊处理以粘附低熔点琼脂糖,避免“脱胶”问题出现

3)      采用特殊的DNA荧光染料,能有效降低背景干扰,更加方便读取实验结果。


彗星实验试剂盒信息:

品名

货号

规格

说明

OxiSelectTM Comet Assay Kit (3-Well Slides)

STA-350

15 assays

试剂盒内有53孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料,共可检测15个样品。

OxiSelectTM Comet Assay Kit (3-Well Slides)

STA-351

75 assays

试剂盒内有25张3孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料,共可检测75个样品。

OxiSelectTM Comet Assay Kit (96-Well Slides)

STA-355

96 assays

试剂盒内有1张96孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料,共可检测96个样品。



为了
满足客户更多样的实验需求,彗星实验试剂盒特殊处理电泳载玻片可以单独购买,详情如下:

 


品名

货号

规格

产品图片

Comet Assay Slides, 3-Well

STA-352

5 slides

STA-353

25 slides

Comet Assay Slides, 96-Well

STA-356

1 slides

STA-356-5

5 slides

产品部分发表文献:


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